JAK2 V617F
Mutation Detection RT-PCR Kit

JAK2 V617F Mutation Detection Kit is a Real-time PCR in vitro diagnostic kit for the detection of the JAK2 (Janus Kinase 2) V617F allele in genomic DNA extracted from EDTA whole blood against a background of wild-type genomic DNA. The mutation observed is a Guanine to Thymidine transversion in position 1849 of the JAK2 gene, which leads to valine to phenylalanine substitution in position 617 of the protein.

Kit Format & Chemistry: Single-Tube, Multiplex Real-Time PCR Assay based on Taqman-probe-based Chemistry
Target: LNA-based for detection of JAK2 (Janus Kinase 2) V617F allele in genomic DNA against a background of Wild Type Allele.
Internal Control: Beta Actin
LoD /Analytical sensitivity: 10 copies/uL
PCR Run time: <90 Minutes
Sample Compatability: DNA extracted from Whole Blood EDTA.
RT-PCR: Open-SystemPlatform; Instruments with FAM, Texas Red/ROX Channels
Standardized: WHO International standards - NIBSC 16/120

Storage & Precautions

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