Genetic disorders are caused due to a change in the DNA, either in whole or in part, away from the normal sequence. Genetic disorders can be caused by a single gene mutation (monogenic disorder), by multiple mutations (multifactorial inheritance disorder), by gene mutations-environmental factors, or by damage to chromosomes (numerical or structural).

 

Real-time PCR is currently one of the most powerful molecular approaches and is widely used in gene expression analysis and mutation detection for robust diagnosis of genetic disorders.