Human Leukocyte Antigen B27 (HLA B27)
RT-PCR Detection Kit for Exon 2/ Exon 3
Human Leukocyte Antigen (HLA), found on White Blood Cells, are proteins that help the body’s immune system tell the difference between its cells and foreign /harmful substances. Although most HLAs protect the body from harm, HLA-B27 is a specific type of protein that contributes to immune system dysfunction.
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SKU
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100 Rxns : NDXIVD005
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50 Rxns : NDXIVD005_01
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20 Rxns : NDXIVD005_02
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CDSCO
Approved
Features & Benefits:
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Kit Format & Chemistry: Single-Tube, Multiplex Real-Time PCR Assay based on Taqman probe-based Chemistry
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High-quality MasterMix with increased Inhibitor Tolerance, Enhanced Thermostability, high specificity, and enables efficient PCR amplification
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Target: Exon 2 & 3 for HLA B27.
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Internal Control: cyp17a1 (Cytochrome P450 17A1)
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LoD /Analytical sensitivity: 10 copies/uL
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PCR Run time: <70 Minutes
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Sample Compatibility: DNA extracted from Whole Blood, Plasma or Serum
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RT-PCR: Open-System Platform
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Compatible Instruments with HEX/VIC, Texas Red/ROX Channels
Storage & Precautions
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Recommended Storage: -20°C
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For Professional Use only.
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Read user manual
Clinical Relevance
HLA-B27 real-time PCR is clinically important for the early and accurate diagnosis of spondyloarthropathies, especially ankylosing spondylitis, where HLA-B27 shows a strong genetic association. It supports differential diagnosis in patients with chronic back pain, uveitis, reactive arthritis, psoriatic arthritis, and inflammatory bowel disease associated arthritis.
Real-time PCR offers high sensitivity, specificity, and rapid turnaround, making it superior to serology for detecting HLA-B27 alleles, including variant subtypes relevant in diverse populations such as India.
