HIV-1 Quantitative
RT-PCR Detection Kit
HIV-1 Quantitative RT-PCR Detection Kit is an in-vitro RT-PCR quantitative assay for the detection and quantification of HIV-1 (Human Immunodeficiency Virus-1) RNA extracted from human plasma, serum, whole blood along with human endogenous control. The intended users of the product are medical and research professionals in diagnostics, laboratories & healthcare.
SKU
100 Rxns : NDXIVD006
50 Rxns : NDXIVD006_01
20 Rxns : NDXIVD006_02
Available on
GEM ID : 5116877-28812228734
Exclusively Distributed By
QIAGEN India Pvt Ltd.
CDSCO
Approved
Features & Benefits:
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Technique: Taqman Chemistry-based RT-PCR
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Kit Format: Single Tube, Multiplex Assay that detects a target gene encoding gag protein of HIV genome.
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Internal Control: Beta Actin
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MasterMix with Increased Inhibitor Tolerance, Enhanced Thermostability, highly specific, efficient PCR amplification, and also supports reverse transcription.
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No additional cDNA preparation steps.
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PCR run time: 90 Minutes.
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Sample Compatability: Whole Blood, Plasma, or Serum.
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Open Platform Kit compatible with RT-PCR instruments with CY5 and Texas Red Channels
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Calibrated with WHO-certified NIBSC standards: 4th WHO International Standard for HIV-1 RNA (NIBSC code: 16/194)
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LoD /Analytical sensitivity: 20 IU/mL, 34.8 Copies/mL
Storage & Precautions
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Recommended Storage: -20°C
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For Professional Use only.
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Read user manual
Clinical Relevance
Quantification of HIV-1 RNA in plasma specimens is considered a 'gold standard' for evaluating the effectiveness of antiretroviral therapy and long-term patient management. This molecular technique allows us to measure the level of viral load and thereby estimate the degree of viral replication. This will provide a baseline for initiating therapy and will also help us to monitor virological suppression to undetectable levels which is a prerequisite to prevent the development of drug-resistant mutations. Additionally, this technique is also essential for the early diagnosis of primary HIV infection during the window period and for the definitive diagnosis of HIV-1 infection in children of HIV positive mothers. In fact, an undetectable viral load is a major clinical endpoint to rule out the risk of sexual transmission, quantitative RT-PCR technique is essential for patient care and surveillance.
